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axiovert 200 inverted microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovert 200 inverted microscope
    Axiovert 200 Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+axiovert+200+microscope/200m+axiovert+microscope/pmc12277648-88-9-13
    Average 90 stars, based on 1 article reviews
    axiovert 200 inverted microscope - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Na + /H + exchanger NHE1 is active at cell-cell contacts and facilitates cell dissemination during collective migration of melanoma cells.
    Article Snippet: .. Before measurements, cell culture medium was replaced by HEPES-buffered RPMI-1640 (SigmaAldrich, St. Louis, USA) and the cells placed in a heating cabinet at 37°C and room air for 3 h. Cell dishes were then placed on the stage of an inverted Axiovert 200 microscope (Carl Zeiss, Oberkochen, Germany) and continuously superfused with different prewarmed (37°C) experimental solutions (see below). .. For dual excitation, wavelengths of 490 and 460 nm were generated with a high-speed polychromator (Visitron Systems, Puchheim, Germany).

    Microscopy:

    Article Title: Na + /H + exchanger NHE1 is active at cell-cell contacts and facilitates cell dissemination during collective migration of melanoma cells.
    Article Snippet: .. Before measurements, cell culture medium was replaced by HEPES-buffered RPMI-1640 (SigmaAldrich, St. Louis, USA) and the cells placed in a heating cabinet at 37°C and room air for 3 h. Cell dishes were then placed on the stage of an inverted Axiovert 200 microscope (Carl Zeiss, Oberkochen, Germany) and continuously superfused with different prewarmed (37°C) experimental solutions (see below). .. For dual excitation, wavelengths of 490 and 460 nm were generated with a high-speed polychromator (Visitron Systems, Puchheim, Germany).

    Article Title: Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP2-mediated endocytosis
    Article Snippet: .. The microscope was based on an inverted Axiovert 200 microscope (Zeiss) and equipped with a spinning disk confocal unit (CSU10; Yokogawa) and triple-band dichroic mirror (Di01-T442/514/647, Semrock). .. Excitations of CFP, YFP, and mCherry were conducted with diode lasers and a semiconductor laser (COHERENT, OBIS 445 nm LX 75 mW, OBIS 514 nm LX 40 mW, OBIS 561 nm LS 50 mW), which were fiber-coupled (OZ optics) to the spinning disk unit.

    Article Title: Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP2-mediated endocytosis.
    Article Snippet: .. The microscope was based on an inverted Axiovert 200 microscope (Zeiss) and equipped with a spinning disk confocal unit (CSU10; Yokogawa) and triple-band dichroic mirror (Di01-T442/514/ 647, Semrock). .. Excitations of CFP, YFP, and mCherry were conducted with diode lasers and a semiconductor laser (COHERENT, OBIS 445 nm LX 75mW, OBIS 514 nm LX 40mW, OBIS 561 nm LS 50mW), which were fiber-coupled (OZ optics) to the spinning disk unit.

    Article Title: Glutamine-rich Regions of the Disordered CREB Transactivation Domain Mediate Dynamic Intra- and Intermolecular Interactions
    Article Snippet: .. This system consists of an inverted Axiovert 200 microscope (Zeiss, Thornwood, NY) that focuses incoming excitation light at 488 nm from a DL488-050-O diode pumped solid state laser (CrystaLaser, Reno, NV) into the sample solution, roughly 30 μm above the glass cover- slip surface, using a water immersion objective (1.2 NA, 63X; Zeiss). ..

    Article Title: Na + /H + exchanger NHE1 is active at cell-cell contacts and facilitates cell dissemination during collective migration of melanoma cells.
    Article Snippet: Non-specific binding sites were blocked with 3% bovine serum albumin (BSA; diluted in PBS; Sigma-Aldrich, St. Louis, USA) at RT for 2 h. For immunodetection, cells were incubated with the monoclonal mouse anti-NHE1 antibody (BD Biosciences Cat# 611775, RRID:AB_399261, diluted 1:100 in 3% BSA-PBS) at RT for 1 h, washed 5× in PBS and then exposed to the secondary goat anti mouse-IgG Alexa 488 antibody (Thermo Fisher Scientific Cat# A-11001, RRID:AB_2534069, diluted 1:1600 in 3% BSA-PBS) at RT for 45 min. Coverslips were mounted with a fluorescence mounting medium containing the DNA stain DAPI (Agilent Technologies, Santa Clara, USA). .. Immunofluorescence images were acquired using an inverted Axiovert 200 microscope (Carl Zeiss, Oberkochen, Germany) equipped with a RT-SE-Spot model 9.0 digital camera (SPOT Imaging, Sterling Heights, USA) and the MetaVue software (version 6.3r6, Molecular Devices, San Jose, USA). ..

    Article Title: Met-Signaling Controls Dendritic Cell Migration in Skin by Regulating Podosome Formation and Function.
    Article Snippet: For motility assay, the remaining one third of the chamber was filled with complete RPMI supplemented with 10% FCS in the presence or absence of 50 ng/ml HGF (eBioscience) and the inhibitors PD98059 or SU11274. .. Bright-field time-lapse recordings of cells within threedimensional collagen gels were acquired at one frame per minute for up to 4 hours using an inverted AxioVert 200 microscope (Zeiss) equipped with a humidified climate chamber (PeCon, Erbach, Germany) adjusted at 5% carbon dioxide, 37 C, and a Cascade 512B EMCCD camera (Photometrics, Tucson, AZ) operated with IPlab Spectrum software (Scanalytix, Wauwatosa, WI). ..

    Article Title: Decisive role of mDia-family formins in cell cortex function of highly adherent cells.
    Article Snippet: .. Briefly, a chamber with one open side was filled with 1 ml of PBS buffer and mounted on a stage of an inverted Axiovert 200 microscope (Zeiss) equipped with an LD Achroplan 40×/0.6 objective (Zeiss). .. A BSAcoated glass micropipette with an inner diameter of 4.6 ± 1.3 μm was also filled with PBS buffer and positioned into the measurement chamber using a micromanipulator (Narishige).

    Article Title: The Anopheles leucine-rich repeat protein APL1C is a pathogen binding factor recognizing Plasmodium ookinetes and sporozoites
    Article Snippet: .. Ookinete microscopy used a high-speed spinning-disk confocal system UltraVIEW ERS (Perkin Elmer) mounted on an inverted Axiovert 200 microscope (Carl Zeiss). ..

    Immunofluorescence:

    Article Title: Na + /H + exchanger NHE1 is active at cell-cell contacts and facilitates cell dissemination during collective migration of melanoma cells.
    Article Snippet: Non-specific binding sites were blocked with 3% bovine serum albumin (BSA; diluted in PBS; Sigma-Aldrich, St. Louis, USA) at RT for 2 h. For immunodetection, cells were incubated with the monoclonal mouse anti-NHE1 antibody (BD Biosciences Cat# 611775, RRID:AB_399261, diluted 1:100 in 3% BSA-PBS) at RT for 1 h, washed 5× in PBS and then exposed to the secondary goat anti mouse-IgG Alexa 488 antibody (Thermo Fisher Scientific Cat# A-11001, RRID:AB_2534069, diluted 1:1600 in 3% BSA-PBS) at RT for 45 min. Coverslips were mounted with a fluorescence mounting medium containing the DNA stain DAPI (Agilent Technologies, Santa Clara, USA). .. Immunofluorescence images were acquired using an inverted Axiovert 200 microscope (Carl Zeiss, Oberkochen, Germany) equipped with a RT-SE-Spot model 9.0 digital camera (SPOT Imaging, Sterling Heights, USA) and the MetaVue software (version 6.3r6, Molecular Devices, San Jose, USA). ..

    Imaging:

    Article Title: Na + /H + exchanger NHE1 is active at cell-cell contacts and facilitates cell dissemination during collective migration of melanoma cells.
    Article Snippet: Non-specific binding sites were blocked with 3% bovine serum albumin (BSA; diluted in PBS; Sigma-Aldrich, St. Louis, USA) at RT for 2 h. For immunodetection, cells were incubated with the monoclonal mouse anti-NHE1 antibody (BD Biosciences Cat# 611775, RRID:AB_399261, diluted 1:100 in 3% BSA-PBS) at RT for 1 h, washed 5× in PBS and then exposed to the secondary goat anti mouse-IgG Alexa 488 antibody (Thermo Fisher Scientific Cat# A-11001, RRID:AB_2534069, diluted 1:1600 in 3% BSA-PBS) at RT for 45 min. Coverslips were mounted with a fluorescence mounting medium containing the DNA stain DAPI (Agilent Technologies, Santa Clara, USA). .. Immunofluorescence images were acquired using an inverted Axiovert 200 microscope (Carl Zeiss, Oberkochen, Germany) equipped with a RT-SE-Spot model 9.0 digital camera (SPOT Imaging, Sterling Heights, USA) and the MetaVue software (version 6.3r6, Molecular Devices, San Jose, USA). ..

    Software:

    Article Title: Met-Signaling Controls Dendritic Cell Migration in Skin by Regulating Podosome Formation and Function.
    Article Snippet: For motility assay, the remaining one third of the chamber was filled with complete RPMI supplemented with 10% FCS in the presence or absence of 50 ng/ml HGF (eBioscience) and the inhibitors PD98059 or SU11274. .. Bright-field time-lapse recordings of cells within threedimensional collagen gels were acquired at one frame per minute for up to 4 hours using an inverted AxioVert 200 microscope (Zeiss) equipped with a humidified climate chamber (PeCon, Erbach, Germany) adjusted at 5% carbon dioxide, 37 C, and a Cascade 512B EMCCD camera (Photometrics, Tucson, AZ) operated with IPlab Spectrum software (Scanalytix, Wauwatosa, WI). ..



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